Open Accessmicro RNA ‐762 promotes breast cancer cell proliferation and invasion by targeting IRF 7 expression
Author(s) -
Li Yongping,
Huang Ruixue,
Wang Ling,
Hao Junsheng,
Zhang Qiong,
Ling Rui,
Yun Jun
Publication year - 2015
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12223
Subject(s) - irf7 , microrna , cell growth , breast cancer , cancer research , cell , cell culture , biology , cancer , gene expression , microbiology and biotechnology , gene , genetics
Objectives mi RNA s play crucial roles in human tumourigenesis. This study was performed to measure expression and function of miR‐762 in breast cancer. Materials and methods Expression of miR‐762 in breast tissues and cell lines ( SK ‐ BR ‐3, DA ‐ MB ‐435s, MCF ‐7 and MDA ‐ MB ‐231, HBL ‐100) was measured by using real‐time RT ‐ PCR . We restored expression of miR‐762 in MCF ‐7 cells to measure its functional roles. Luciferase assays were performed to reveal the target gene of miR‐762. Results Expression of miR‐762 was high in both breast cancer cell lines and specimens, and its overexpression increased breast cancer cell proliferation and invasion. Interferon regulatory factor 7 ( IRF 7) is a direct target of miR‐762 and overexpression of miR‐762 reduced expression of IRF 7. Moreover, IRF 7 was repressed, its levels inversely correlated to miR‐762 expression. IRF 7 rescued miR‐762‐induced cell invasion and proliferation. Conclusions These results demonstrate that miR‐762 tumour effect was achieved by targeting IRF 7 in human breast cancer specimens.