Open AccessPrognostic value of circulating CD133 + cells in patients with gastric cancer
Author(s) -
Xia Pu,
Song ChangLiang,
Liu JinFang,
Wang Dan,
Xu XiaoYan
Publication year - 2015
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12175
Subject(s) - clonogenic assay , abcg2 , cancer , in vivo , flow cytometry , cancer stem cell , cancer cell , in vitro , cancer research , immunofluorescence , biology , pathology , medicine , immunology , microbiology and biotechnology , antibody , biochemistry , atp binding cassette transporter , transporter , gene
Abstract Objectives Gastric cancer is an important cause of cancer‐related mortality worldwide (1). There is increasing evidence that the existence of cancer stem cells ( CSC ) is responsible for tumour formation and maintenance. Materials and methods The present study was designed to recognise circulating CSC s from blood samples of patients with gastric cancer, using CD 133 and ABCG 2 as potential markers. CD133 − , CD133 + ABCG2 − and CD133 + ABCG2 + cells lines were analysed by flow cytometry, immunofluorescence staining, western blotting and real‐time PCR . Furthermore, functional assays (clonogenic assay in vitro and tumourigenic assay in vivo ) were also performed using these cell lines. Results Higher percentages of CD133 + cells were identified in blood samples from gastric cancer patients compared to normal controls. In addition, we found by using Kaplan–Meier analysis, that numbers of CD133 + cells correlated with poor prognosis gastric cancer patients. Finally, tumourigenic properties of CD133 + ABCG2 + cells were determined in vitro and in vivo . Conclusions Our in vitro and in vivo experiments demonstrated that CD133 + ABCG2 + cells exhibited well‐known CSC characteristics; thus when circulating they could be used as a prognostic marker for gastric cancer.