Open AccessPorphyromonas gingivalis lipopolysaccharides regulate functions of bone marrow mesenchymal stem cells
Author(s) -
Tang J.,
Wu T.,
Xiong J.,
Su Y.,
Zhang C.,
Wang S.,
Tang Z.,
Liu Y.
Publication year - 2015
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12173
Subject(s) - porphyromonas gingivalis , mesenchymal stem cell , periodontitis , stem cell , lipopolysaccharide , regeneration (biology) , immunology , bone marrow , microbiology and biotechnology , chemistry , biology , medicine
Objectives Periodontitis is one of the most widespread inflammatory diseases; it causes tooth loss and is also associated with a variety of systemic diseases. Mesenchymal stem cells ( MSC s) have been used to treat periodontitis. However, it is unknown whether bacterial toxins in the periodontal environment affect MSC ‐mediated periodontal regeneration. Porphyromonas gingivalis lipopolysaccharides ( Pg ‐LPS) are key toxins for development of periodontitis. The purpose of the present study was to investigate effects of P. gingivalis LPS on biological properties of MSC s. Materials and methods Mesenchymal stem cells from bone marrow ( BMMSC s) were treated with different concentrations of P. gingivalis LPS (0.1–10 μg/ml), then its effects were evaluated on biological properties of BMMSC s including proliferation, apoptosis, osteogenic differentiation and capacities to inhibit activated T cells. Results Low concentration of P. gingivalis LPS (0.1 μg/ml) accelerated MSC proliferation, osteogenic differentiation and capacities to inhibit activated T cells via up‐regulation of nitric oxide. However, high concentration of P. gingivalis LPS (10 μg/ml) reduced MSC proliferation, osteogenic differentiation and capacities to inhibit activated T cells. Conclusions Mesenchymal stem cells were functionally different following exposure to P. gingivalis LPS at the investigated concentrations. These findings suggest that MSC ‐mediated periodontal regeneration may be regulated by P. gingivalis LPS .