Open AccessInducing pluripotency in vitro : recent advances and highlights in induced pluripotent stem cells generation and pluripotency reprogramming
Author(s) -
Rony I. K.,
Baten A.,
Bloomfield J. A,
Islam M. E.,
Billah M. M.,
Islam K. D.
Publication year - 2015
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12162
Subject(s) - reprogramming , induced pluripotent stem cell , sox2 , klf4 , regenerative medicine , biology , embryonic stem cell , somatic cell , microbiology and biotechnology , epigenetics , stem cell , cell , genetics , gene
Abstract Induced pluripotent stem cells ( iPSC s) are considered patient‐specific counterparts of embryonic stem cells as they originate from somatic cells after forced expression of pluripotency reprogramming factors Oct4, Sox2, Klf4 and c‐Myc. iPSC s offer unprecedented opportunity for personalized cell therapies in regenerative medicine. In recent years, iPSC technology has undergone substantial improvement to overcome slow and inefficient reprogramming protocols, and to ensure clinical‐grade iPSC s and their functional derivatives. Recent developments in iPSC technology include better reprogramming methods employing novel delivery systems such as non‐integrating viral and non‐viral vectors, and characterization of alternative reprogramming factors. Concurrently, small chemical molecules (inhibitors of specific signalling or epigenetic regulators) have become crucial to iPSC reprogramming; they have the ability to replace putative reprogramming factors and boost reprogramming processes. Moreover, common dietary supplements, such as vitamin C and antioxidants, when introduced into reprogramming media, have been found to improve genomic and epigenomic profiles of iPSC s. In this article, we review the most recent advances in the iPSC field and potent application of iPSC s, in terms of cell therapy and tissue engineering.