Open AccessEffects of anodic titanium dioxide nanotubes of different diameters on macrophage secretion and expression of cytokines and chemokines
Author(s) -
Lü W. L.,
Wang N.,
Gao P.,
Li C. Y.,
Zhao H. S.,
Zhang Z. T.
Publication year - 2015
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12149
Subject(s) - chemokine , secretion , macrophage , nanotube , adhesion , macrophage inflammatory protein , materials science , cytokine , microbiology and biotechnology , interleukin 8 , inflammation , tumor necrosis factor alpha , titanium , proinflammatory cytokine , biophysics , in vitro , chemistry , carbon nanotube , nanotechnology , biology , immunology , biochemistry , composite material , metallurgy
Objectives To investigate effects of TiO 2 nanotubes of different diameters on J744A.1 macrophage behaviour, secretion and expression of pro‐inflammatory cytokines and chemokines. Materials and methods Macrophage‐like J744A.1 cells were cultured on three types of Ti surface: mechanically polished titanium plus 30 and 80 nm TiO 2 nanotube surfaces, for 4, 24 and 48 h. Macrophage adhesion and proliferation were assessed using CCK ‐8 assay. Levels of pro‐inflammatory cytokines ( TNF ‐α, IL ‐1β and IL ‐6) and chemokines ( MCP ‐1 and MIP ‐1α) secreted into the supernatant were measured using the Cytometric Bead Arrays kit. TNF ‐ α , MCP ‐1 and MIP ‐1 α gene expression were quantitatively analysed by real‐time PCR . Results These show that TiO 2 nanotube surfaces, especially of 80 nm TiO 2 nanotube, benefited macrophage adhesion and proliferation, and reduced protein secretion and mRNA expression of TNF‐α, MCP‐1 and MIP‐1α. IL‐1β and IL‐6 were undetectable on all the surfaces at all times. Conclusions TiO 2 nanotube surfaces, especially of 80 nm TiO 2 nanotube, reduced inflammatory response in vitro , which might be part of a basis for rapid osseointegration in implants with TiO 2 nanotube surfaces in animal studies.