Open AccessEffects of conservatively treated diseased cementum with or without EMD on in vitro cementoblast differentiation and in vivo cementum‐like tissue formation of human periodontal ligament cells
Author(s) -
Qi Y.,
Feng W.,
Cai J.,
Sun Q.,
Li S.,
Li M.,
Song A.,
Yang P.
Publication year - 2014
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12116
Subject(s) - cementum , cementoblast , periodontal fiber , dental cementum , cementogenesis , chemistry , dentin , bone sialoprotein , in vivo , enamel matrix derivative , in vitro , anatomy , dentistry , microbiology and biotechnology , biology , osteocalcin , alkaline phosphatase , medicine , biochemistry , regeneration (biology) , enzyme
Objectives The present study aimed to evaluate the effects of conservatively treated diseased cementum on in vitro cementoblast differentiation and in vivo cementum‐like tissue formation of human periodontal ligament cells ( hPDLC s), and observe differential effects of enamel matrix derivative ( EMD ) on in vivo cementum formation by hPDLC s. Materials and methods Forty‐eight cementum slices and 48 dentin slices were prepared from periodontitis compromised teeth, and hPDLC s were inoculated on to all root slices. Twenty‐four co‐cultured root slices of each group were used for mRNA expression of cementum attachment protein and CEMP1. With application of EMD , 24 co‐cultured root slices (divided into groups C, D, C+E, D+E) were transplanted subcutaneously into nude mice. All root fragments were reviewed by histological analysis and immunohistochemical staining for bone sialoprotein. Results mRNA expressions of cementum attachment protein and cementum protein ‐ 1 from hPDLC s on cementum slices were statistically higher than those of dentin slices. Seven specimens of group C and 10 specimens of group C+E revealed a layer of cementum‐like tissue ( NFC ) on surfaces of pre‐existing cementum. NFC was thicker in group C+E than in group C. All NFC s were positively stained for bone sialoprotein, however, there was no NFC formation on dentin slices. Conclusion Conservatively treated diseased cementum promoted in vitro cementoblast differentiation and in vivo cementum‐like tissue formation by hPDLC s, and the in vivo effect was enhanced by the presence of EMD .