Open AccessDifferential characteristics and in vitro angiogenesis of bone marrow‐ and peripheral blood‐derived endothelial progenitor cells: evidence from avian species
Author(s) -
Shah Q. A.,
Tan X.,
Bi S.,
Liu X.,
Hu S.
Publication year - 2014
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12111
Subject(s) - progenitor cell , bone marrow , matrigel , biology , peripheral blood mononuclear cell , in vitro , microbiology and biotechnology , vasculogenesis , stem cell , angiogenesis , immunology , andrology , cancer research , medicine , biochemistry
Objectives This study was conducted to compare phenotypes and in vitro angiogenic capacity of putative endothelial progenitor cells ( EPC s) derived from bone marrow ( BM ) and peripheral blood ( PB ), from an avian species. Materials and methods Mononuclear cells were isolated from chicken BM and PB ( BMMNC s and PBMNC s) and cultured in EGM ‐2 medium. Cells at days 7–14 were used for the experiments. Expression of progenitor and endothelial markers, number of Dil–ac– LDL /lectin dual‐positive cells and adipogenic and osteogenic differentiation were determined. Migration and in vitro angiogenic ability between BMMNC ‐ and PBMNC ‐derived cells were compared. Results PBMNC s developed typical EPC appearance, with initial spindle shape followed by a cobblestone form, whereas BMMNC ‐derived cells retained their constitutive spindle‐like morphology throughout the study. Cells derived from both sources expressed CD 133, CD 31 and VEGFR ‐2, although PBMNC s‐derived cells had lower CD 133 expression. Nevertheless, number of Dil–ac– LDL /lectin dual‐positive cells did not differ between groups. Adipogenic and osteogenic lineages were verified in BMMNC ‐ but not in PBMNC ‐derived cells. PBMNC ‐derived cells formed tubular networks on Matrigel. However, BMMNC ‐derived cells formed few tube‐like structures, which were not morphologically comparable to those developed by their counterparts. Conclusion Our results suggest that so called EPC s derived from BMMNC s are not ‘true’ EPC s, supporting previous findings on mammals that BM may not serve as an optimal isolation source of EPC s.