Open AccessSurface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro‐hydroxyapatite/titanium oxide coating on titanium surface by sol–gel method
Author(s) -
He G.,
Guo B.,
Wang H.,
Liang C.,
Ye L.,
Lin Y.,
Cai X.
Publication year - 2014
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12105
Subject(s) - coating , materials science , titanium , biocompatibility , osseointegration , scanning electron microscope , substrate (aquarium) , layer (electronics) , apatite , anatase , composite material , chemical engineering , crystallization , oxide , metallurgy , implant , chemistry , photocatalysis , engineering , medicine , biochemistry , oceanography , surgery , geology , catalysis
Objectives To improve efficacy of current titanium and its alloys, in bioactivity and speed of osseointegration, of orthopaedic implants. Materials and methods A novel triple‐layered functional graded coating, consisting of a porous hydroxyapatite ( HA ) outermost layer, fluoro‐HA ( FHA ) intermediate layer and titanium oxide (TiO 2 ) innermost layer, was created on a titanium substrate by a multistep sol–gel method. X‐ray diffraction analysis showed TiO 2 anatase and apatite crystallization in the coating. Results Morphological analysis performed by scanning electron microscopy showed excellent bonding between coating and substrate, with a thickness of ~2 μm. Scratch testing found favourable adhesion strength of the composite coating. In addition, optical microscope images suggested good biocompatibility. Considering thet in vitro cell response, osteoblasts on the coating exhibited higher cell proliferation and ALP activity compared to pure titanium and HA coating, and demonstrated excellent coating bioactivity. Conclusions Current results indicated that the novel TiO 2 / FHA / HA coating has promising clinical applications in orthopaedic and dental implantation.