Open AccessInduction of apoptosis and G 2 /M cell cycle arrest by Scrophularia striata in a human leukaemia cell line
Author(s) -
Azadmehr A.,
Hajiaghaee R.,
Mazandarani M.
Publication year - 2013
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12074
Subject(s) - propidium iodide , apoptosis , jurkat cells , cell cycle , cell culture , flow cytometry , annexin , mtt assay , cell cycle checkpoint , cytotoxic t cell , biology , cell growth , traditional medicine , programmed cell death , microbiology and biotechnology , biochemistry , t cell , immunology , medicine , in vitro , genetics , immune system
Objectives Scrophularia striata Boiss (Scrophulariaceae) is a plant that grows in northeastern Iran; it has been used traditionally to treat various inflammatory disorders. This study was designed to investigate cytotoxic effects of S. striata extract, on the Jurkat human leukaemia cell line (T‐cell leukaemia). Materials and methods Phytochemical assay by thin layer chromatography and 2, 2 diphenyl‐1‐picryl‐hydrazyl were used to evaluate main compounds and antioxidant capacity of the plant extract, respectively. Its inhibitory effect on Jurkat cells was evaluated by MTT assay. In addition, cell cycle distribution and apoptotic cell death were evaluated by propidium iodide and annexin V‐FITC/ propidium iodide staining. Results These showed that the main components present in S. striata extract included flavonoids, phenolic compounds and phenyl propanoids. Treatment with extract was significantly cytotoxic to the tumour cell line. In addition, flow cytometry analysis indicated that S. striata extract induced cell cycle arrest in G 2 /M phase and apoptosis of tumour cells. Conclusions Results of the study indicated that S. striata extract could inhibit leukaemia cell proliferation by inducing G 2 /M phase arrest and apoptosis.