Open AccessSerum‐free media for the production of human mesenchymal stromal cells: a review
Author(s) -
Gottipamula S.,
Muttigi M. S.,
Kolkundkar U.,
Seetharam R. N.
Publication year - 2013
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12063
Subject(s) - bovine spongiform encephalopathy , mesenchymal stem cell , context (archaeology) , fetal bovine serum , stromal cell , population , immunology , biology , in vitro , microbiology and biotechnology , medicine , pathology , cancer research , environmental health , disease , genetics , prion protein , paleontology
The regenerative potential of mesenchymal stromal cells ( MSC ) holds great promise in using them for treatment of a wide range of debilitating diseases. Several types of culture media and systems have been used for large‐scale expansion of MSC s in vitro; however, the majority of them rely heavily on using foetal bovine serum ( FBS )‐supplement for optimal cell proliferation. FBS ‐based cultures pose the potential threat of spread of transmissible spongiform encephalopathy and bovine spongiform encephalopathy to MSC s and then to their recipients. A recent trend in cell culture is to change from serum‐use to serum‐free media ( SFM ). In this context, the current review focuses specifically on employment of various SFM for MSC s and discusses existences of various options with which to substitute FBS . In addition, we analyse MSC population growth kinetic patterns using various SFM for large‐scale production of MSC s.