Open AccessMechanical compressive force inhibits adipogenesis of adipose stem cells
Author(s) -
Li G.,
Fu N.,
Yang X.,
Li M.,
Ba K.,
Wei X.,
Fu Y.,
Yao Y.,
Cai X.,
Lin Y.
Publication year - 2013
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12053
Subject(s) - adipogenesis , oil red o , adipose tissue , in vitro , chemistry , messenger rna , staining , microbiology and biotechnology , biomedical engineering , biology , biochemistry , gene , pathology , medicine
Objective The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose‐derived stem cells ( ASC s) in vitro . Materials and methods Mice ASC s were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet‐filled cells. Adipogenic genes, PPAR ‐γ1 and APN , were examined by real‐time PCR and immunofluorescence ( IF ) staining was performed to test expression of de‐ PPAR ‐γ and ph‐ PPAR ‐γ at the protein level. Results Our data showed that mechanical compressive force reduced numbers of oil droplet‐filled cells, and down‐regulated mRNA levels of both PPAR‐γ1 and APN and protein level of PPAR‐γ, in ASCs. Conclusions In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASC s.