Open AccessInterleukin‐6‐induced satellite cell proliferation is regulated by induction of the JAK 2/ STAT 3 signalling pathway through cyclin D1 targeting
Author(s) -
Kurosaka M.,
Machida S.
Publication year - 2013
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12045
Subject(s) - stat , cyclin d1 , jak stat signaling pathway , microbiology and biotechnology , cell growth , signalling , signal transduction , cell , biology , cell cycle , stat3 , genetics , tyrosine kinase
Objectives To determine whether interleukin‐6 ( IL ‐6) stimulates rat muscle satellite cell proliferation in culture, and if so, to clarify the signalling mechanisms. Materials and methods Primary satellite cells were isolated from thirty male F344 rats, 11 weeks of age. IL ‐6 at concentrations of 0.01, 0.1, 1, 10 or 100 ng/ml was added to culture media. Results IL‐6 at 0.01–1 ng/ml induced dose‐dependent increase in cell proliferation. After treatment with 1 ng/ml IL‐6, cell proliferation increased by 31%, and p‐STAT3 + /MyoD + cells increased in number compared to those in control media ( P < 0.05). Inhibitors of JAK2 (AG 490) and STAT3 (STAT3 peptide) blocked the increase in BrdUrd + cell numbers at 6 h post stimulation with 1 ng/ml IL‐6 ( P < 0.05). Furthermore, cyclin D1 mRNA expression and cyclin D1 + /MyoD + cell numbers significantly increased in cultures treated with 1 ng/ml IL‐6 compared to those in control media ( P < 0.05). In contrast, treatment with 10 and 100 ng/ml IL‐6 did not stimulate cell proliferation. Treatment with 10 ng/ml IL‐6 induced greater SOCS3 mRNA expression than with 1 ng/ml IL‐6 and control media. Moreover, co‐localization of SOCS3 and myogenin was observed after treatment with 10 ng/ml IL‐6. Conclusions IL ‐6 induced dose‐dependent increase in satellite cell proliferation by activating the JAK 2/ STAT 3/cyclin D1 pathway.