Open AccessLow‐intensity pulsed ultrasound induced enhanced adipogenesis of adipose‐derived stem cells
Author(s) -
Fu N.,
Yang X.,
Ba K.,
Fu Y.,
Wei X.,
Yue Y.,
Li G.,
Yao Y.,
Chen J.,
Cai X.,
Liang C.,
Ge Y.,
Lin Y.
Publication year - 2013
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12031
Subject(s) - adipogenesis , low intensity pulsed ultrasound , adipose tissue , in vitro , chemistry , messenger rna , stem cell , microbiology and biotechnology , staining , andrology , therapeutic ultrasound , ultrasound , biology , gene , medicine , pathology , biochemistry , radiology
Objective The aim of this study was to investigate effects of low‐intensity pulsed ultrasound ( LIPUS ) on differentiation of adipose‐derived stem cells ( ASC s), in vitro . Materials and methods Murine ASC s were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR ‐γ1 , and APN , was examined by real‐time PCR . Immunofluorescence ( IF ) staining was performed to test for PPAR‐γ at the protein level. Results Our data revealed that specific patterns of LIPUS up‐regulated levels of both PPAR‐γ1 and APN mRNA , and PPAR‐γ protein. Conclusions In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.