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Choline treatment affects the liver reticuloendothelial system and plasma fatty acid composition in diabetic rats
Author(s) -
AlSaeedi Fatma J.,
Cheng Behling
Publication year - 2013
Publication title -
clinical physiology and functional imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.608
H-Index - 67
eISSN - 1475-097X
pISSN - 1475-0961
DOI - 10.1111/cpf.12027
Subject(s) - medicine , endocrinology , palmitoleic acid , choline , oleic acid , linoleic acid , fatty acid , arachidonic acid , fatty liver , chemistry , biochemistry , disease , enzyme
Summary Purpose This study investigated effects of choline treatment on hepatic reticuloendothelial and biliary functions and plasma fatty acid composition in diabetic rats. Methods Diabetes was induced by streptozotocin ( STZ ). Choline was administered to untreated rats and a portion of STZ ‐treated rats for two sequences of five consecutive days, separated by a 2‐day interval. Hepatic functions were studied using 99m Tc Tin ( II ) colloid ( TIN ) and 99 mTc mebrofenin [bromo‐iminodiacetic acid (Br IDA )] imaging. The TIN ‐uptake ratios (organ/whole body) of heart, liver and spleen, and the Br IDA ‐uptake ratios (organ or tissue/whole body) of liver, biliary tree and abdomen were obtained following imaging studies. Fatty acids were analysed by GC / MS . Results Choline treatment did not attenuate hyperglycaemic development. Diabetic rats showed (i) a decreased TIN ‐uptake ratio in liver with co‐increased ratios in heart and spleen; choline treatment diminished these changes, (ii) elevated Br IDA ‐uptake ratios in biliary tree and abdomen but not in liver; choline treatment did not attenuate the elevations and (iii) decreases in plasma palmitoleic acid and oleic acid, reflecting an impaired stearoyl‐CoA desaturase function; choline treatment did not affect the diminutions, but caused a decrease in arachidonic acid with a co‐increase in linoleic acid. Some rats developed hypoproteinemia ( HPO ). HPO rats also exhibited decreases in plasma palmitoleic acid and oleic acid. Diabetes caused almost absence of palmitoleic acid in HPO rats. Choline treatment exerted no effect on the plasma fatty acid composition of diabetic HPO rats. Conclusions Choline treatment affected hepatic reticuloendothelial function and plasma fatty acid composition, but not hepatobiliary function, in diabetic rats. Whether choline treatment is beneficial requires further studies.