Premium
A new colorimetric assay for antioxidant capacity and photostability
Author(s) -
FernándezGarcía Elisabet,
HeluaniGahete Hayat,
Wellinger Ralf E.
Publication year - 2016
Publication title -
coloration technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 49
eISSN - 1478-4408
pISSN - 1472-3581
DOI - 10.1111/cote.12210
Subject(s) - methylene blue , chemistry , antioxidant , ascorbic acid , glutathione , cysteine , antioxidant capacity , acetylcysteine , colorimetry , chromatography , biochemistry , food science , catalysis , enzyme , photocatalysis
The antioxidant capacity of cysteine, glutathione, N ‐acetylcysteine, and ascorbic acid to reduce Methylene Blue to colourless Leucomethylene Blue was analysed. Interestingly, transient exposure of Leucomethylene Blue to sunlight resulted in a reversible oxidation reaction and thus, provided a fast, easy, and simple quantitative assay to determine antioxidant capacity and photostability of biologically relevant antioxidants.