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Bright fluorescent ds DNA probes: novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine‐quinolinium chromophore
Author(s) -
Vasilev Aleksey,
Lesev Nedyalko,
Dimitrova Sonya,
NedelchevaVeleva Marina,
Stoynov Stoyno,
Angelova Silvia
Publication year - 2015
Publication title -
coloration technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 49
eISSN - 1478-4408
pISSN - 1472-3581
DOI - 10.1111/cote.12133
Subject(s) - cyanine , chromophore , fluorescence , chemistry , dna , intercalation (chemistry) , monomer , photochemistry , pyridinium , biochemistry , organic chemistry , optics , physics , polymer
Seven tri‐ and tetracationic monomeric and homodimeric monomethine cyanine dyes based on thiazolo[4,5 ‐b ]pyridinium and quinolinium end groups were synthesised and characterised. The dyes were tested as fluorescent DNA intercalating probes to apply in DNA gel electrophoresis. The DNA samples stained with all dyes from the series demonstrated bright fluorescent signals. DNA fragments were successfully visualised under orange and green filters as well as under standard UV transillumination. Two of the studied dyes revealed higher sensitivity to DNA when compared with the commercial dimeric cyanine dye TOTO ‐1. Their sensitivity reached that of the commercial dimeric cyanine dye YOYO ‐1, but the emission was shifted to longer wavelengths. These qualities make the dyes suitable to apply in a wide range of medical and scientific analytical methods.