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Peripheral blood leucocytes show differential expression of tumour progression‐related genes in colorectal cancer patients who have a postoperative intra‐abdominal infection: a prospective matched cohort study
Author(s) -
Alonso S.,
Mayol X.,
ll L.,
Salvans S.,
Pascual M.,
Pera M.
Publication year - 2017
Publication title -
colorectal disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.029
H-Index - 89
eISSN - 1463-1318
pISSN - 1462-8910
DOI - 10.1111/codi.13635
Subject(s) - medicine , colorectal cancer , microarray , prospective cohort study , immune system , angiogenesis , microarray analysis techniques , downregulation and upregulation , fold change , immunology , gene , cancer , gastroenterology , pathology , gene expression , biochemistry , chemistry
Aim Anastomotic leak is associated with higher rates of recurrence after surgery for colorectal cancer. However, the mechanisms responsible are unknown. We hypothesized that the infection‐induced inflammatory response may induce overexpression of tumour progression‐related genes in immune cells. The aim was to investigate the effect of postoperative intra‐abdominal infection on the gene expression patterns of peripheral blood leucocytes ( PBL ) after surgery for colorectal cancer. Method Prospective matched cohort study. Patients undergoing surgery for colorectal cancer were included. Patients who had anastomotic leak or intra‐abdominal abscess were included in the infection group ( n = 23) and matched with patients without complications for the control group ( n = 23). PBL were isolated from postoperative blood samples. Total RNA was extracted and hybridized to the Affymetrix Human Gene 1.0 ST microarray. Results Patients in the infection group displayed 162 upregulated genes and 146 downregulated genes with respect to the control group. Upregulated genes included examples coding for secreted cytokines involved in tumour growth and invasion ( S100P , HGF , MMP 8 , MMP 9 , PDGFC , IL 1R2 ). Infection also upregulated some proangiogenic genes ( CEP 55 , TRPS 1 ) and downregulated some inhibitors of angiogenesis ( MME , ALOX 15 , CXCL 10 ). Finally, some inhibitors ( HP , ORM 1 , OLFM 4 , IRAK 3 ) and activators ( GNLY , PRF 1 , FGFBP 2 ) of antitumour immunity were upregulated and downregulated, respectively, suggesting that the inflammatory environment caused by a postoperative infection favours immune evasion mechanisms of the tumour. Conclusion Analysis of PBL shows differential expression of certain tumour progression‐related genes in colorectal cancer patients who have a postoperative intra‐abdominal infection, which in turn may promote the growth of residual cancer cells to become recurrent tumours.