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In vivo assessment of cytological changes by means of reflectance confocal microscopy – demonstration of the effect of topical vitamin E on skin irritation caused by sodium lauryl sulfate
Author(s) -
Casari Alice,
Farnetani Francesca,
De Pace Barbara,
Losi Amanda,
Pittet JeanChristophe,
Pellacani Giovanni,
Longo Caterina
Publication year - 2017
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/cod.12613
Subject(s) - irritation , confocal microscopy , confocal , reflectivity , in vivo , chemistry , skin irritation , dermatology , pathology , medicine , biology , optics , immunology , physics , microbiology and biotechnology
Summary Background Irritant contact dermatitis is caused by skin barrier damage. Vitamin E is an antioxidant that is commonly used in cosmetics to prevent photo‐damage. Objectives To show the usefulness of reflectance confocal microscopy in the assessment of irritant skin damage caused by sodium lauryl sulfate ( SLS ) and of the protective action of vitamin E applied prior to skin irritation. Methods Ten healthy volunteers were enrolled. Irritation was induced by the application of a patch test containing SLS 5% aq. for 24 h. Three sites were compared: one site on which a product with vitamin E was applied before SLS treatment, one site on which the same product was applied after SLS treatment, and one control site ( SLS only). Each site was evaluated with reflectance confocal microscopy, providing in vivo tissue images at nearly histological resolution. We also performed a computerized analysis of the V iva S tack® images. Results Reflectance confocal microscopy is able to identify signs of skin irritation and the preventive effect of vitamin E application. Conclusion Reflectance confocal microscopy is useful in the objective assessment of irritative skin damage.