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Patch testing with serial dilutions and thin‐layer chromatograms of oak moss absolutes containing high and low levels of atranol and chloroatranol
Author(s) -
Mowitz Martin,
Zimerson Erik,
Svedman Cecilia,
Bruze Magnus
Publication year - 2013
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/cod.12126
Subject(s) - serial dilution , moss , chromatography , chemistry , absolute (philosophy) , botany , medicine , biology , philosophy , alternative medicine , epistemology , pathology
Summary Background Oak moss absolute ( Evernia prunastri extract) contains a large number of substances, among them the potent allergens atranol and chloroatranol. Since 2008, their content in oak moss absolute has been restricted by the I nternational F ragrance A ssociation to a maximum level of 100 ppm each. Objectives To compare the elicitation capacities of a traditional (sample A ) and a treated (sample B ) oak moss absolute containing, in total, 27 000 and 66 ppm of atranol and chloroatranol, respectively, and to investigate reactions to components of oak moss absolute separated by thin‐layer chromatography ( TLC ). Methods Fifteen oak moss‐allergic subjects were patch tested with serial dilutions and TLC strips of samples A and B . Results Fifteen subjects reacted to sample A at concentrations ≤ 2.0%, and 2 subjects reacted to sample B at 2.0% but not to lower concentrations. Among 13 subjects reacting to the TLC strip of sample A , 11 reacted to spots with retardation factor values corresponding to those of atranol and/or chloroatranol, and 11 reacted to other areas on the TLC strip. Only one subject reacted to the TLC strip of sample B . Conclusions The patch test reactivity of sample B was significantly lower than that of sample A . The TLC patch tests indicate the presence of sensitizers other than atranol and chloroatranol in oak moss absolute.