Premium
CD4 + T cells producing interleukin (IL)‐17, IL‐22 and interferon‐ γ are major effector T cells in nickel allergy
Author(s) -
DyringAndersen Beatrice,
Skov Lone,
Løvendorf Marianne B.,
Bzorek Michael,
Søndergaard Knud,
Lauritsen JensPeter H.,
Dabelsteen Sally,
Geisler Carsten,
Menné Bonefeld Charlotte
Publication year - 2013
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/cod.12043
Subject(s) - immunology , allergy , t lymphocyte , interleukin 4 , nickel allergy , interleukin 2 , cytokine , medicine , effector , interleukin 12 , interleukin 5 , interleukin , cytotoxic t cell , biology , immune system , contact dermatitis , in vitro , biochemistry
Summary Background It has been suggested that interleukin (IL)‐17 and IL‐22 play important roles in the elicitation of human allergic contact dermatitis; however, the frequencies of T cell subtypes producing IL‐17 and IL‐22 in human allergic contact dermatitis are unknown. Objectives To determine the frequencies of CD4 + , CD8 + and γδ T cells producing IL‐17, IL‐22 and interferon (IFN)‐ γ in the blood and skin from nickel‐allergic patients. Patients/materials/methods Blood samples were collected from 14 patients and 17 controls, and analysed by flow cytometry. Biopsies were taken from 5 patients and 6 controls, and analysed by immunohistochemistry and flow cytometry of skin lymphocytes. Results We found an increased frequency of γδ T cells in the blood, but no differences in the distribution of cytokine‐producing CLA + T cell subtypes in nickel‐allergic patients as compared with controls. In nickel‐allergic patients, there was massive cellular infiltration dominated by CD4 + T cells producing IL‐17, IL‐22 and IFN‐ γ in nickel‐challenged skin but not in vehicle‐challenged skin. Conclusion CD4 + T cells producing IL‐17, IL‐22 and IFN‐ γ are important effector cells in the eczematous reactions of nickel‐induced allergic contact dermatitis in humans.