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Characterization of the T cell response in allergic contact dermatitis caused by corticosteroids
Author(s) -
Baeck Marie,
Soria Angèle,
Marot Liliane,
Theate Ivan,
Hendrickx Emilie,
Van Belle Astrid,
Goossens An,
Tennstedt Dominique,
Dachelet Claire,
Jaeger Johnathan,
Pilette Charles,
Renauld JeanChristophe,
Van Baren Nicolas,
Rozières Aurore,
Nicolas JeanFançois,
Dumoutier Laure
Publication year - 2013
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/cod.12040
Subject(s) - cd8 , cytotoxic t cell , allergic contact dermatitis , immunology , cd3 , cytokine , t cell , t cell receptor , allergy , medicine , interleukin 4 , antigen , immune system , biology , in vitro , biochemistry
Summary Background Delayed allergic hypersensitivity reactions have classically been described as type IV reactions, which are caused by T cells; however, the respective roles of CD4 + and CD8 + cells are yet to be defined. A central role for CD8 + cytotoxic T cells as effector cells has been suggested. Objectives To determine the type of T cell involved in corticosteroid allergy. Methods We analysed the kinetics of T cell recruitment and the cytokine production profile in positive patch tests of 27 corticosteroid‐sensitized patients, as compared with control sites and control subjects. Skin biopsies, collected at 8, 24 and 48 hr following drug application, were embedded in paraffin for histological and immunohistological staining, and, in some cases, also deep‐frozen for gene expression analyses. Results CD3 + T cells were rapidly recruited in concert with the positivity of the patch test sites. High levels of interleukin (IL)‐4, IL‐5 and, to a lesser extent, interferon‐ γ suggested that both Th2 and Th1 cytokines were implicated. IL‐4 was also produced by γδ T cell receptor (TCR) lymphocytes. Conclusions This study showed that, in allergic contact dermatitis caused by corticosteroids, the inflammatory infiltrate is composed of CD3 + T cells with a predominant Th2 cytokine profile, among which IL‐4 is also produced by γδ TCR lymphocytes.