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The transcriptome characteristics of vestibular organs from delayed endolymphatic hydrops patients (Meniere’s disease)
Author(s) -
Chen BinJun,
Luo Wenwei,
Zhao WeiDong,
Qian XiaoQing,
Wang Yanmei,
Zheng Yu,
Wang XinWei,
Xu Xinda,
Yuan YaSheng,
Chi FangLu,
Ren DongDong
Publication year - 2021
Publication title -
clinical otolaryngology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.914
H-Index - 68
eISSN - 1749-4486
pISSN - 1749-4478
DOI - 10.1111/coa.13748
Subject(s) - endolymphatic hydrops , medicine , transcriptome , vestibular system , meniere's disease , acoustic neuroma , gene expression , gene , disease , pathology , audiology , genetics , biology
Objectives To identify genes that are related to delayed endolymphatic hydrops (DEH) in patients by RNA‐Seq analysis. Design Observational study. Setting Eye & ENT Hospital, Fudan University (Shanghai, China). Participants We collected the entire vestibular system from four patients with DEH who underwent labyrinthectomy. Three control samples were collected from patients with acoustic neuroma or facial neuroma treated via the translabyrinthine approach. High‐throughput RNA‐Seq analysis was performed to investigate gene expression in the pathological vestibular system. Main outcome measures Our bioinformatic analysis identified 17 genes that were upregulated and eight genes that were downregulated in patients with DEH compared with the controls. Results The altered gene expression profile suggested that DEH is closely related to neuropathy and autoimmune disease. In addition, many of the differentially regulated genes were involved in cell adhesion, suggesting a role of cell adhesion in DEH. Immunofluorescence analysis confirmed the expression of PMP2 and CLDN19 in the cytoplasm of hair cells and scattered expression of MPZ at cell junctions. The protein expression levels were higher in specimens from patients with Ménière's disease and DEH compared with controls. Conclusions The protein expression profile of vestibular organs in patients with endolymphatic hydrops exhibited a degree of similarity to that of Ménière's disease. Endolymphatic hydrops is characterised by autoimmune abnormalities. DEH and Ménière's disease are likely to be different manifestations of the same disease, with disparate clinical symptoms. RNA‐Seq is a useful analytical tool to characterise the vestibular pathology based on its transcriptome.