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Using a semi‐conductor sequencing‐based panel for genotyping of HPV ‐positive and HPV ‐negative oropharyngeal cancer: a retrospective pilot study
Author(s) -
Ham J.C.,
Tops B.B.J.,
Driessen C.M.L.,
van Raaij A.W.M.,
Slootweg P.J.,
Melchers W.J.G.,
Ligtenberg M.J.L.,
van Herpen C.M.L.
Publication year - 2017
Publication title -
clinical otolaryngology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.914
H-Index - 68
eISSN - 1749-4486
pISSN - 1749-4478
DOI - 10.1111/coa.12800
Subject(s) - medicine , genotyping , mutation , tyrosine kinase , gene , receptor tyrosine kinase , oncology , polymerase chain reaction , cancer research , genotype , receptor , genetics , biology
Objectives The aim of this study was to assess the feasibility of testing actionable mutations in small amounts of formalin‐fixed paraffin‐embedded material in multiple genes of the receptor tyrosine kinase pathway and to determine the frequency of these mutations in human papillomavirus ( HPV )‐positive and HPV ‐negative oropharyngeal cancer ( OPC ). Design A retrospective pilot study was performed. Setting In OPC , no predictive markers for response to epidermal growth factor receptor inhibition are known. Therefore, identifying predictive biomarkers is of utmost importance, but is often hampered by the small amount of tumour material available. Participants We included the archival material of 45 OPC , all treated with concomitant chemoradiotherapy between 2003 and 2010. Main outcome measures Besides the HPV status, we assessed mutations using a gene panel that targets 16 genes in the receptor tyrosine kinase pathway and six other genes. The polymerase chain reaction required only 10 ng DNA . Results In total, 42 of the 45 biopsies have been successfully analysed. In total 20 of 42 samples were HPV ‐positive and 22 of 42 were HPV ‐negative. In the receptor tyrosine kinase pathway, mutations in PIK 3 CA were most frequently identified. A TP 53 mutation was identified in one HPV ‐positive sample and in 13 HPV ‐negative samples. Additionally, three mutations in three different genes were found. Conclusions We evaluated an assay to identify mutations in the receptor tyrosine kinase pathway. As only small amounts of formalin‐fixed paraffin‐embedded material are sufficient for reliable analysis, this test opens up new possibilities for personalised medicine.