Open AccessMemory improvement in senile rats after prebiotic and probiotic supplementation is not induced by GLP ‐1
Author(s) -
ServínCasas Gabriela Andrea,
RomoAraiza Alejandra,
GutierrezSalmean Gabriela,
MartinezSolis Enrique,
IbarraGarcía Andrea Paola,
CruzMartinez Yolanda,
RodriguezBarrera Roxana,
García Elisa,
IncontriAbraham Diego,
Ibarra Antonio
Publication year - 2022
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.13951
Subject(s) - prebiotic , probiotic , inulin , enterococcus faecium , morris water navigation task , food science , hippocampus , biology , chemistry , biochemistry , endocrinology , genetics , bacteria , antibiotics
The mechanism underlying the memory improvement induced by prebiotic and probiotic supplementation remains unclear. Glucagon‐like peptide type 1 (GLP‐1) could play an important role since it is induced by prebiotics and enhances memory and learning. Aims We correlated the levels of GLP‐1 with spatial memory in senile animals to determine its role in memory improvement after prebiotic and probiotic supplementation. Methods Senile rats were randomly assigned to four groups: (1) water (control); (2) Enterococcus faecium (probiotic); (3) agave inulin (prebiotic); and (4) E. faecium + agave inulin (symbiotic). Each supplement was administered by an orogastric cannula for 5 weeks. In the fifth week, spatial memory was assessed using the Morris Water Maze test (MWM). We extracted the hippocampus, intestine, and serum. GLP‐1 levels were quantified by enzyme‐linked immunosorbent assay. Results A significant decrease in escape latency time in the MWM was observed in all groups treated with supplements. The symbiotic group achieved the highest reduction (15.13 s ± 6.40) ( p < 0.01). We did not find a significant increase in GLP‐1 levels nor a direct correlation of its levels with spatial memory improvement ( p > 0.05). Conclusion Prebiotic and probiotic supplementation improved spatial memory in senile animals. However, this beneficial effect did not correlate with GLP‐1 levels.