Open AccessPronounced antiepileptic activity of the subtype‐selective GABA A ‐positive allosteric modulator PF‐06372865 in the GAERS absence epilepsy model
Author(s) -
Duveau Venceslas,
Buhl Derek L.,
Evrard Alexis,
Ruggiero Céline,
MandéNiedergang Betty,
Roucard Corinne,
Gurrell Rachel
Publication year - 2019
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.13046
Subject(s) - epilepsy , allosteric modulator , allosteric regulation , gabaa receptor , pharmacology , medicine , diazepam , neuroscience , receptor , anticonvulsant , anesthesia , psychology , psychiatry
Summary Aim Antiepileptic drugs that modulate GABA have the potential to aggravate or improve the symptoms of absence epilepsy. PF‐06372865 is a positive allosteric modulator (PAM) of α2/3/5 subunit‐containing GABA A receptors with minimal activity at α1‐containing receptors, which are believed to mediate many of the adverse events associated with benzodiazepines. The aim of this study was to assess the antiepileptic effect of PF‐06372865 in a preclinical model of absence seizures. Methods Genetic absence epilepsy rats from Strasbourg (GAERS) was implanted with four cortical electrodes over the frontoparietal cortex, and the number and cumulated duration of spike‐and‐wave discharges (SWDs) were recorded for 10‐90 minutes following administration of vehicle, PF‐06372865, and positive controls diazepam and valproate. Results PF‐06372865 (0.3, 1, 2, 10 mg kg −1 ) dose‐dependently reduced the expression of SWDs, including full suppression at the highest doses by 30 minutes after administration. Conclusions PF‐06372865 demonstrated robust efficacy in suppressing SWDs in the GAERS model of absence epilepsy. To our knowledge, this is the first demonstration of antiepileptic activity of an α2/3/5‐subtype‐selective GABA A PAM in a model of absence epilepsy. Further study of the antiepileptic properties of PF‐06372865 is warranted in patients with absence seizures.