Open AccessRetracted: Micro RNA ‐21 promotes glioma cell proliferation and inhibits senescence and apoptosis by targeting SPRY 1 via the PTEN / PI 3K/ AKT signaling pathway
Author(s) -
Chai Chang,
Song LaiJun,
Han ShuangYin,
Li XiQing,
Li Ming
Publication year - 2018
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.12785
Subject(s) - pten , pi3k/akt/mtor pathway , protein kinase b , apoptosis , glioma , cancer research , chemistry , cell growth , caspase 3 , microbiology and biotechnology , biology , programmed cell death , biochemistry
Summary Aims Our study aims to investigate the effect of micro RNA ‐21 (miR‐21) on the proliferation, senescence, and apoptosis of glioma cells by targeting SPRY 1 via the PTEN / PI 3K/ AKT signaling pathway. Methods Glioma tissues and brain tissues were collected for this study after surgical decompression for traumatic brain injury. RT ‐ qPCR was employed to measure mRNA levels of miR‐21, SPRY 1, PTEN , PI 3K, and AKT , and Western blotting was conducted to determine protein levels of SPRY 1, PTEN , PI 3K, AKT , p‐ AKT , Caspase‐3, Caspase‐9, P53, GSK 3, and p‐ GSK 3. Human glioma U87 cells were assigned into the blank, negative control ( NC ), miR‐21 mimics, miR‐21 inhibitors, si RNA ‐ SPRY 1, and miR‐21 inhibitors + si RNA ‐ SPRY 1 groups, with human HEB cells serving as the normal group. Cell proliferation, cell cycle, and apoptosis were determined by MTT and flow cytometry, respectively. Results Compared with control group, an increased expression of miR‐21, PI 3K, AKT , p‐ AKT , P53, and p‐ GSK 3, and a decreased expression of SPRY 1, PTEN , Caspase‐3, and Caspase‐9 were observed in the glioma group, and no significant differences were found in the expression of GSK 3. SPRY 1 was verified to be the target gene of miR‐21. Compared with the blank and NC groups, levels of PI 3K, AKT , p‐ AKT , P53, and p‐ GSK 3 increased while levels of SPRY 1, PTEN , Caspase‐3, and Caspase‐9 decreased in the miR‐21 mimics and si RNA ‐ SPRY 1 groups; the miR‐21 inhibitors group reversed the tendency; furthermore, the miR‐21 inhibitors group showed decreased cell proliferation but promoted apoptosis, which were opposite to the results of the miR‐21 mimics and si RNA ‐ SPRY 1 groups. Conclusion Micro RNA ‐21 might promote cell proliferation and inhibit cell senescence and apoptosis of human glioma cells by targeting SPRY 1 via the PTEN / PI 3K/ AKT signaling pathway.