Open AccessNeuroprotective Efficacy of an Aminopropyl Carbazole Derivative P7C3‐A20 in Ischemic Stroke
Author(s) -
Wang ShuNa,
Xu TianYing,
Wang Xia,
Guan YunFeng,
Zhang SaiLong,
Wang Pei,
Miao ChaoYu
Publication year - 2016
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.12576
Subject(s) - neuroprotection , pharmacology , medicine , ischemia , chemistry , anesthesia
Summary Aim NAMPT is a novel therapeutic target of ischemic stroke. The aim of this study was to investigate the effect of a potential NAMPT activator, P7C3‐A20, an aminopropyl carbazole derivative, on ischemic stroke. Methods In vitro study, neuron protection effect of P7C3‐A20 was investigated by co‐incubation with primary neurons subjected to oxygen–glucose deprivation (OGD) or oxygen–glucose deprivation/reperfusion (OGD/R) injury. In vivo experiment, P7C3‐A20 was administrated in middle cerebral artery occlusion (MCAO) rats and infarct volume was examined. Lastly, the brain tissue nicotinamide adenine dinucleotide (NAD) levels were detected in P7C3‐A20 treated normal or MCAO mice. Results Cell viability, morphology, and Tuj‐1 staining confirmed the neuroprotective effect of P7C3‐A20 in OGD or OGD/R model. P7C3‐A20 administration significantly reduced cerebral infarction in MCAO rats. Moreover, brain NAD levels were elevated both in normal and MCAO mice after P7C3‐A20 treatment. Conclusions P7C3‐A20 has neuroprotective effect in cerebral ischemia. The study contributes to the development of NAMPT activators against ischemic stroke and expands the horizon of the neuroprotective effect of aminopropyl carbazole chemicals.