Open AccessMicro RNA ‐29c Correlates with Neuroprotection Induced by FNS by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke
Author(s) -
Huang LiGang,
Li JinPin,
Pang XiaoMin,
Chen ChunYong,
Xiang HuiYao,
Feng LingBo,
Su ShengYou,
Li ShengHua,
Zhang Lei,
Liu JingLi
Publication year - 2015
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.12383
Subject(s) - neuroprotection , messenger rna , rna , gene expression , microrna , western blot , microbiology and biotechnology , biology , neuroscience , chemistry , medicine , gene , biochemistry
Summary Aims Studies showed fastigial nucleus stimulation ( FNS ) reduced brain damage, but the mechanisms of neuroprotection induced by FNS were not entirely understood; Micro RNA s are noncoding RNA molecules that regulate gene expression in a posttranscriptional manner, but their functional consequence in response to ischemia–reperfusion ( IR ) remains unknown. We investigated the role of micro RNA ‐29c in the neuroprotection induced by FNS in rat. Methods The IR rat models were conducted 1 day after FNS . Besides, miR‐29c antagomir (or agomir or control) was infused to the left intracerebroventricular 1 day before IR models were conducted. We detected differential expression of Birc2 mRNA (also Bak1m RNA and miR‐29c) level among different groups by RT ‐ qPCR . The differential expression of Birc2 protein (also Bak1 protein) level among different groups was surveyed via Western blot. The neuroprotective effects were assessed by infarct volume, neurological deficit, and apoptosis. Results MiR‐29c was decreased after FNS . Moreover, miR‐29c directly bound to the predicted 3′‐ UTR target sites of Birc2 and Bak1 genes. Furthermore, over‐expression of miR‐29c effectively reduced Birc2 (also Bak1) mRNA and protein levels, increased infarct volume and apoptosis, and deteriorated neurological outcomes, whereas down‐regulation played a neuroprotective role. Conclusions MiR‐29c correlates with the neuroprotection induced by FNS by negatively regulating Birc2 and Bak1.