Open AccessRegenerative Therapy in Experimental Parkinsonism: Mixed Population of Differentiated Mouse Embryonic Stem Cells, Rather Than Magnetically Sorted and Enriched Dopaminergic Cells Provide Neuroprotection
Author(s) -
Tripathy Debasmita,
Verma Poonam,
NthengeNgumbau Dominic N.,
Banerjee Meghna,
Mohanakumar Kochupurackal P.
Publication year - 2014
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.12295
Subject(s) - dopaminergic , striatum , substantia nigra , tyrosine hydroxylase , population , dopamine , embryonic stem cell , transplantation , biology , neurotrophic factors , neuroscience , endocrinology , medicine , biochemistry , receptor , environmental health , gene
Summary Aim The objective of the study was to develop regenerative therapy by transplanting varied populations of dopaminergic neurons, differentiated from mouse embryonic stem cells ( mES ) in the striatum for correcting experimental parkinsonism in rats. Methods mES differentiated by default for 7 days in serum‐free media (7D), or by enhanced differentiation of 7D in retinoic acid (7R), or dopaminergic neurons enriched by manual magnetic sorting from 7D (SSEA−) were characterized and transplanted in the ipsilateral striatum of 6‐hydroxydopamine‐induced hemiparkinsonian rats. Neurochemical, neuronal, glial and neurobehavioral recoveries were examined. Results 7R and SSEA− contained significantly reduced NANOG and high MAP2 mRNA and protein levels as revealed, respectively, by reverse transcriptase‐PCR and immunocytochemistry, compared with 7D. Striatal engraftment of 7D resulted in a significantly better behavioral and neurochemical recovery, as compared to the animals that received either 7R or SSEA−. The 7R transplanted animals showed improvement neither in behavior nor in striatal dopamine level. The grafted striatum revealed increased GFAP staining intensity in 7D and SSEA−, but not in 7R cells transplanted group, suggesting a vital role played by glial cells in the recovery. Substantia nigra ipsilateral to the side of the striatum, which received transplants showed more tyrosine hydroxylase immunostained neurons, as compared to 6‐hydroxydopamine‐infused animals. Conclusion These results demonstrate that default differentiated mixed population of cells are better than sorted, enriched dopaminergic cells, or cells containing more mature neurons for transplantation recovery in hemiparkinsonian rats.