Open AccessThe Suppressive Effect of IL ‐27 on Encephalitogenic Th17 Cells Induced by Multiwalled Carbon Nanotubes Reduces the Severity of Experimental Autoimmune Encephalomyelitis
Author(s) -
Moraes Adriel S.,
Paula Rosemeire F.O.,
Pradella Fernando,
Santos Mariana P.A.,
Oliveira Elaine C.,
Glehn Felipe,
Camilo Daniela S.,
Ceragioli Helder,
Peterlevitz Alfredo,
Baranauskas Vitor,
Volpini Walkyria,
Farias Alessandro S.,
Santos Leonilda M.B.
Publication year - 2013
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.12121
Subject(s) - experimental autoimmune encephalomyelitis , interleukin 17 , adoptive cell transfer , antigen presenting cell , rar related orphan receptor gamma , chemistry , t cell , immunology , microbiology and biotechnology , immune system , biology , foxp3
Summary Background Both Th1 and Th17 cells specific for neuroantigen are described as encephalitogenic in the experimental autoimmune encephalomyelitis ( EAE ) model. Aim The proposal of this study was to investigate how carbon nanotubes internalized by antigen‐presenting cells (APCs) affect the development of encephalitogenic CD 4 + T cells. Methods Therefore, we stimulated encephalitogenic T cells in the presence or not of multiwalled carbon nanotube ( MWCNT ). After the incubation, we analyzed the expression profile of the encephalitogenic T cells and their capacity to induce EAE . Results Encephalitogenic CD 4 + T cells cultured with APC s that were previously incubated with MWCNT s do not express IL ‐17. The adoptive transfer of these cells causes less severe EAE than the transfer of both Th1 and Th17 cells that are not incubated with MWCNT s. These results suggest that the increased IL ‐27 level produced by the APC s incubated with the carbon nanotubes inhibits the development of Th17 cells. This observation is confirmed by the concomitant reduction in the level of ROR γt, which is a transcription factor essential for the development of Th17 cells. Moreover, the incubation of encephalitogenic T cells devoid of Th17 cells with neutralizing anti‐ IL ‐27 antibodies restored the production of IL ‐17. Conclusion This finding confirms the suppressive effect of IL ‐27 on encephalitogenic Th17 cells. The results presented suggest that the stimulation of APC s with carbon nanoparticles prior to neuroantigen presentation affects the development of the Th17 subset of encephalitogenic CD 4 + T lymphocytes and results in less severe EAE .