Open AccessPifithrin‐α Enhances the Survival of Transplanted Neural Stem Cells in Stroke Rats by Inhibiting p53 Nuclear Translocation
Author(s) -
Lei XuHui,
Zhao Dan,
Li YongLi,
Li XianFeng,
Sun Xu,
Du WenZhong,
Sun Ying,
Hao ZhongFei,
Xin ShiYue,
Liu Cong,
Zhang ZhiRen,
Jiang ChuanLu
Publication year - 2013
Publication title -
cns neuroscience and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 69
eISSN - 1755-5949
pISSN - 1755-5930
DOI - 10.1111/cns.12045
Subject(s) - neural stem cell , tunel assay , apoptosis , transplantation , chromosomal translocation , viability assay , cell , biology , chemistry , stem cell , cancer research , microbiology and biotechnology , medicine , biochemistry , gene
Summary Aims To examine a novel strategy to enhance the survival of grafted neural stem cells ( NSC s) in stroke model. Methods Using a cell counting kit‐8 ( CCK ‐8) and TUNEL assay to test the protective effects of p53 inhibitor, pifithrin‐α ( PFT ‐α), on oxygen glucose deprivation ( OGD ) in NSC s. We compared the effects of vehicle + NSC s and FFT ‐α + NSC s on the efficacy of transplantation in stroke rat model using behavioral analysis, immunohistochemistry, etc. Results Pifithrin‐α increased viability and decreased apoptosis in NSC s after OGD in vitro . By in vivo studies, we showed that the best recovery of neurological function in the stroke rats and the maximum survival of grafted NSC s were found in the PFT ‐α + NSC s group. Twelve hours after cell transplantation, p53 was localized to the nuclei of grafted NSC s in the vehicle + NSC s group but was primarily localized to the cytoplasm in the PFT ‐α + NSC s group. The p53‐upregulated modulator of apoptosis ( PUMA ) was highly expressed among the grafted cells in the vehicle + NSC s group compared with that in the PFT ‐α + NSC s group. Conclusion Our results indicate that PFT ‐α enhances the survival of grafted NSC s through the inhibition of p53 translocation into the nucleus.