Open AccessSalmonella exploits host Rho GTPase signalling pathways through the phosphatase activity of SopB
Author(s) -
Truong Dorothy,
Boddy Kirsten C.,
Canadien Veronica,
Brabant Danielle,
Fairn Gregory D.,
D'Costa Vanessa M.,
Coyaud Etienne,
Raught Brian,
PérezSala Dolores,
Park Wei Sun,
Heo Won Do,
Grinstein Sergio,
Brumell John H.
Publication year - 2018
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12938
Subject(s) - effector , biology , rhob , microbiology and biotechnology , salmonella , gtpase , secretion , type three secretion system , virulence , signal transduction , gene , genetics , bacteria , biochemistry , rhoa
Salmonella uses Type 3 secretion systems (T3SSs) to deliver virulence factors, called effectors, into host cells during infection. The T3SS effectors promote invasion into host cells and the generation of a replicative niche. SopB is a T3SS effector that plays an important role in Salmonella pathogenesis through its lipid phosphatase activity. Here, we show that SopB mediates the recruitment of Rho GTPases (RhoB, RhoD, RhoH, and RhoJ) to bacterial invasion sites. RhoJ contributes to Salmonella invasion, and RhoB and RhoH play an important role in Akt activation. R‐Ras1 also contributes to SopB‐dependent Akt activation by promoting the localised production of PI(3,4)P 2 /PI(3,4,5)P 3 . Our studies reveal new signalling factors involved in SopB‐dependent Salmonella infection.