Open AccessTyrosine phosphorylation modulates mitochondrial chaperonin Hsp60 and delays rotavirus NSP4‐mediated apoptotic signaling in host cells
Author(s) -
Chattopadhyay Shiladitya,
Mukherjee Arpita,
Patra Upayan,
Bhowmick Rahul,
Basak Trayambak,
Sengupta Shantanu,
ChawlaSarkar Mamta
Publication year - 2017
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12670
Subject(s) - biology , hsp60 , phosphorylation , tyrosine phosphorylation , tyrosine , heat shock protein , microbiology and biotechnology , mitochondrion , virology , hsp70 , biochemistry , gene
Phosphoproteomics‐based platforms have been widely used to identify post translational dynamics of cellular proteins in response to viral infection. The present study was undertaken to assess differential tyrosine phosphorylation during early hours of rotavirus (RV) SA11 infection. Heat shock proteins (Hsp60) were found to be enriched in the data set of RV‐SA11 induced differentially tyrosine‐phosphorylated proteins at 2 hr post infection (hpi). Hsp60 was further found to be phosphorylated by an activated form of Src kinase on 227th tyrosine residue, and tyrosine phosphorylation of mitochondrial chaperonin Hsp60 correlated with its proteasomal degradation at 2–2.5hpi. Interestingly, mitochondrial Hsp60 positively influenced translocation of the rotaviral nonstructural protein 4 to mitochondria during RV infections. Phosphorylation and subsequent transient degradation of mitochondrial Hsp60 during early hours of RV‐SA11 infection resulted in inhibition of premature import of nonstructural protein 4 into mitochondria, thereby delaying early apoptosis. Overall, the study highlighted one of the many strategies rotavirus undertakes to prevent early apoptosis and subsequent reduced viral progeny yield.