Open AccessBiosynthesis of cell wall mannan in the conidium and the mycelium of Aspergillus fumigatus
Author(s) -
Henry Christine,
Fontaine Thierry,
Heddergott Christoph,
Robinet Pauline,
Aimanianda Vishukumar,
Beau Remi,
Beauvais Anne,
Mouyna Isabelle,
Prevost MarieChristine,
Fekkar Arnaud,
Zhao Yanan,
Perlin David,
Latgé JeanPaul
Publication year - 2016
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12665
Subject(s) - mannan , aspergillus fumigatus , cell wall , biology , mycelium , mannose , microbiology and biotechnology , yeast , galactomannan , aspergillosis , conidium , biochemistry , polysaccharide , botany
The galactomannan is a major cell wall molecule of Aspergillus fumigatus . This molecule is composed of a linear mannan with a repeating unit composed of four α1,6 and α1,2 linked mannose with side chains of galactofuran. To obtain a better understanding of the mannan biosynthesis in A. fumigatus , it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing α1,6 and α1,2 mannose linkages in yeast. These deletions did not lead to a reduction of the mannan content of the cell wall of the mycelium of A. fumigatus . In contrast, the mannan content of the conidial cell wall was reduced and this reduction was associated with a partial disorganization of the cell wall leading to defects in conidial survival both in vitro and in vivo.