z-logo
open-access-imgOpen Access
Discovery of a novel and conserved P lasmodium falciparum exported protein that is important for adhesion of PfEMP 1 at the surface of infected erythrocytes
Author(s) -
Nacer Adéla,
Claes Aurélie,
Roberts Amy,
ScheidigBenatar Christine,
Sakamoto Hiroshi,
Ghorbal Mehdi,
LopezRubio JoseJuan,
Mattei Denise
Publication year - 2015
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12430
Subject(s) - biology , plasmodium falciparum , virulence , gene , complementation , subtelomere , adhesion , cell adhesion , mutant , microbiology and biotechnology , genetics , chromosome , cell , immunology , malaria , chemistry , organic chemistry
Summary P lasmodium falciparum virulence is linked to its ability to sequester in post‐capillary venules in the human host. P lasmodium falciparum erythrocyte membrane protein 1 ( PfEMP 1) is the main variant surface antigen implicated in this process. Complete loss of parasite adhesion is linked to a large subtelomeric deletion on chromosome 9 in a number of laboratory strains such as D 10 and T 9‐96. Similar to the cytoadherent reference line FCR 3, D 10 strain expresses PfEMP 1 on the surface of parasitized erythrocytes, however without any detectable cytoadhesion. To investigate which of the deleted subtelomeric genes may be implicated in parasite adhesion, we selected 12 genes for D 10 complementation studies that are predicted to code for proteins exported to the red blood cell. We identified a novel single copy gene ( PF 3 D 7_0936500) restricted to P . falciparum that restores adhesion to CD 36, termed here virulence‐associated protein 1 ( Pfvap1 ). Protein knockdown and gene knockout experiments confirmed a role of PfVAP 1 in the adhesion process in FCR 3 parasites. PfVAP 1 is co‐exported with PfEMP 1 into the host cell via vesicle‐like structures called M aurer's clefts. This study identifies a novel highly conserved parasite molecule that contributes to parasite virulence possibly by assisting PfEMP 1 to establish functional adhesion at the host cell surface.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here