Open AccessS almonella modulation of the phagosome membrane, role of S se J
Author(s) -
Kolodziejek Anna M.,
Miller Samuel I.
Publication year - 2015
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12420
Subject(s) - phagosome , biology , effector , endosome , rhoa , microbiology and biotechnology , secretion , cytoplasm , intracellular , vacuole , gtpase , type three secretion system , small gtpase , biochemistry , signal transduction , virulence , gene
Summary Salmonellae have the ability to invade, persist and replicate within an intracellular phagosome termed the Salmonella ‐containing vacuole ( SCV ). Salmonellae alter lipid and protein content of the SCV membrane and manipulate cytoskeletal elements in contact with the SCV using the Salmonella pathogenicity island 1 ( SPI ‐2) type III secretion system effectors. These modifications result in microtubular‐based movement and morphological changes, which include endosomal tubulation of the SCV membrane. S se J is a SPI ‐2 effector that localizes to the cytoplasmic face of the SCV and esterifies cholesterol through its glycerophospholipid : cholesterol acyltransferase activity. S se J enzymatic activity as well as localization to the SCV are determined by binding to the small mammalian GTP ase, R ho A . This review will focus on current knowledge about the role of S se J in SCV membrane modification and will discuss how the hypothesis that a major role of SPI ‐2 effectors is to modify SCV protein and lipid content to promote bacterial intracellular survival.