Open AccessLive F aecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier
Author(s) -
Ulluwishewa Dulantha,
Anderson Rachel C.,
Young Wayne,
McNabb Warren C.,
Baarlen Peter,
Moughan Paul J.,
Wells Jerry M.,
Roy Nicole C.
Publication year - 2015
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12360
Subject(s) - faecalibacterium prausnitzii , obligate anaerobe , biology , microbiology and biotechnology , obligate , bacteroidaceae , anaerobic exercise , anaerobic bacteria , cell culture , caco 2 , bacteroides thetaiotaomicron , bacteroides , bacteria , feces , genetics , physiology , ecology
Summary F aecalibacterium prausnitzii , an abundant member of the human commensal microbiota, has been proposed to have a protective role in the intestine. However, it is an obligate anaerobe, difficult to co‐culture in viable form with oxygen‐requiring intestinal cells. To overcome this limitation, a unique apical anaerobic model of the intestinal barrier, which enabled co‐culture of live obligate anaerobes with the human intestinal cell line C aco‐2, was developed. C aco‐2 cells remained viable and maintained an intact barrier for at least 12 h, consistent with gene expression data, which suggested C aco‐2 cells had adapted to survive in an oxygen‐reduced atmosphere. Live F. prausnitzii cells, but not ultraviolet ( UV )‐killed F . prausnitzii , increased the permeability of mannitol across the epithelial barrier. Gene expression analysis showed inflammatory mediators to be expressed at lower amounts in C aco‐2 cells exposed to live F . prausnitzii than UV ‐killed F . prausnitzii , This, consistent with previous reports, implies that live F . prausnitzii produces an anti‐inflammatory compound in the culture supernatant, demonstrating the value of a physiologically relevant co‐culture system that allows obligate anaerobic bacteria to remain viable.