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Summary  Malaria is caused by obligate intracellular parasites, of which   P   lasmodium falciparum  is the most lethal species. In humans,   P   . falciparum  merozoites (invasive forms of the parasite) employ a host of parasite proteins to rapidly invade erythrocytes. One of these is the   P   . falciparum  apical membrane antigen 1 ( PfAMA 1) which forms a complex with rhoptry neck proteins at the tight junction. Here, we have placed the   Pfama1   gene under conditional control using dimerizable  Cre  recombinase ( DiCre ) in   P   . falciparum .  DiCre ‐mediated excision of the  loxP ‐flanked   Pfama1   gene results in approximately 80% decreased expression of the protein within one intraerythrocytic growth cycle. This reduces growth by 40%, due to decreased invasion efficiency characterized by a post‐invasion defect in sealing of the parasitophorous vacuole. These results show that  PfAMA 1 is an essential protein for merozoite invasion in   P   . falciparum  and either directly or indirectly plays a role in resealing of the red blood cell at the posterior end of the invasion event.

Conditional expression of apical membrane antigen 1 in P lasmodium falciparum shows it is required for erythrocyte invasion by merozoites