Open AccessIron‐regulated surface determinant B ( IsdB ) promotes S taphylococcus aureus adherence to and internalization by non‐phagocytic human cells
Author(s) -
Zapotoczna Marta,
Jevnikar Zala,
Miajlovic Helen,
Kos Janko,
Foster Timothy J.
Publication year - 2013
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12097
Subject(s) - internalization , staphylococcus aureus , biology , fibronectin , integrin , bacterial adhesin , microbiology and biotechnology , hela , bacteria , hek 293 cells , cell culture , escherichia coli , receptor , biochemistry , genetics , gene , extracellular matrix
Summary S taphylococcus aureus is a human pathogen that causes invasive and recurring infections. The ability to internalize into and persist within host cells is thought to contribute to infection. Here we report a novel role for the well‐characterized iron‐regulated surface determinant B ( IsdB ) protein which we have shown can promote adhesion of 293 T , HeLa cells and platelets to immobilized bacteria independently of its ability to bind haemoglobin. IsdB bound to the active form of the platelet integrin α IIb β 3 , both on platelets and when the integrin was expressed ectopically in CHO cells. IsdB also promoted bacterial invasion into human cells. This was clearly demonstrated with bacteria lacking fibronectin‐binding proteins ( FnBPs ), which are known to promote invasion in the presence of fibronectin. However, IsdB also contributed significantly to invasion by cells expressing FnBPs in the presence of serum. Thus IsdB appears to be able to interact with the broader family of integrins that bind ligands with the RGD motif and to act as a back up mechanism to promote interactions with mammalian cells.