Open AccessDiversion of phagosome trafficking by pathogenic R hodococcus equi depends on mycolic acid chain length
Author(s) -
Sydor Tobias,
Bargen Kristine,
Hsu FongFu,
Huth Gitta,
Holst Otto,
Wohlmann Jens,
Becken Ulrike,
Dykstra Tobias,
Söhl Kristina,
Lindner Buko,
Prescott John F.,
Schaible Ulrich E.,
Utermöhlen Olaf,
Haas Albert
Publication year - 2013
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/cmi.12050
Subject(s) - phagolysosome , mycolic acid , biology , microbiology and biotechnology , mutant , phagosome , bacteria , mycobacterium , biochemistry , phagocytosis , gene , genetics
Summary R hodococcus equi is a close relative of M ycobacterium spp. and a facultative intracellular pathogen which arrests phagosome maturation in macrophages before the late endocytic stage. We have screened a transposon mutant library of R . equi for mutants with decreased capability to prevent phagolysosome formation. This screen yielded a mutant in the gene for β‐ketoacyl‐(acyl carrier protein)‐synthase A ( KasA ), a key enzyme of the long‐chain mycolic acid synthesizing FAS‐II system. The longest kasA mutant mycolic acid chains were 10 carbon units shorter than those of wild‐type bacteria. Coating of non‐pathogenic E . coli with purified wild‐type trehalose dimycolate reduced phagolysosome formation substantially which was not the case with shorter kasA mutant‐derived trehalose dimycolate. The mutant was moderately attenuated in macrophages and in a mouse infection model, but was fully cytotoxic.Whereas loss of KasA is lethal in mycobacteria, R . equi kasA mutant multiplication in broth was normal proving that long‐chain mycolic acid compounds are not necessarily required for cellular integrity and viability of the bacteria that typically produce them. This study demonstrates a central role of mycolic acid chain length in diversion of trafficking by R . equi .