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In vivo evaluation of shark teeth‐derived bioapatites
Author(s) -
LópezÁlvarez Miriam,
Vigo Eva,
RodríguezValencia Cosme,
OuteiriñoIglesias Verónica,
González Pío,
Serra Julia
Publication year - 2017
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12934
Subject(s) - fluorapatite , apatite , osseointegration , bioceramic , bone mineral , in vivo , chemistry , materials science , whitlockite , implant , dentistry , biomedical engineering , mineralogy , osteoporosis , biology , composite material , pathology , medicine , surgery , microbiology and biotechnology
Objective The present work proposes the shark teeth as a new source of bioapatites for bone filler applications in maxillary sinus elevation, periodontal regeneration or implants placement. This abundant fishing by‐product provides an improved hydroxyapatite ( HA ) with fluorine contributions. The in vivo evaluation of osteointegration and bone mineral density levels promoted by these marine bioapatites was the main objective. Materials and Methods Marine bioapatite granules of two sizes (1 mm, <20 μm) were obtained and characterized ( XRD , SEM , ICP ‐ OES ) to determine morphology and composition. In vivo evaluation was performed, after bioapatites implantation in critical defects of parietal bone of 25 rats, for 3 weeks. Commercial synthetic HA /β TCP (60/40%) material and unfilled defects were used as controls. Radiology, micro‐ CT , histology and quantification of bone mineral density are presented. Results These marine bioapatites presented a globular porous morphology. A biphasic composition ~70% apatitic ( HA , apatite‐CaF, fluorapatite) and ~30% non‐apatitic phase (whitlockite, tricalcium bis(orthophosphate)), with contributions of F (1.0 ± 0.5%wt), Na (0.9 ± 0.2%wt) and Mg (0.65 ± 0.04%wt) was confirmed. After implantation period, higher osteointegration of 1‐mm marine bioapatites than commercial synthetic granules was observed, together with bone formation from the defect surroundings but also at central area (potential osteoinductive properties). New bone cells penetrated inside pores and inter‐granular cavities. Higher bone mineral density, in both 1‐mm and <20‐μm granules, than on commercial synthetic graft was determined, being significant in 1‐mm bioapatites (a P < 0.05). Conclusion Shark teeth bioapatites were successfully validated as new functionally efficient bone filler in rat model, promoting significantly increased bone mineral density than synthetic control.