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Experimental conical‐head abutment screws on the microbial leakage through the implant‐abutment interface: an in vitro analysis using target‐specific DNA probes
Author(s) -
Pita Murillo S.,
Nascimento Cássio,
Santos Carla G. P.,
Pires Isabela M.,
Pedrazzi Vinícius
Publication year - 2017
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12876
Subject(s) - conical surface , abutment , implant , dentistry , materials science , dental abutments , biomedical engineering , orthodontics , composite material , medicine , structural engineering , surgery , engineering
Objectives The aim of this in vitro study was to identify and quantify up to 38 microbial species from human saliva penetrating through the implant‐abutment interface in two different implant connections, external hexagon and tri‐channel internal connection, both with conventional flat‐head or experimental conical‐head abutment screws. Material and methods Forty‐eight two‐part implants with external hexagon ( EH ; n = 24) or tri‐channel internal ( TI ; n = 24) connections were investigated. Abutments were attached to implants with conventional flat‐head or experimental conical‐head screws. After saliva incubation, Checkerboard DNA – DNA hybridization was used to identify and quantify up to 38 bacterial colonizing the internal parts of the implants. Kruskal–Wallis test followed by Bonferroni's post‐tests for multiple comparisons was used for statistical analysis. Results Twenty‐four of thirty‐eight species, including putative periodontal pathogens, were found colonizing the inner surfaces of both EH and TI implants. Peptostreptococcus anaerobios ( P = 0.003), Prevotella melaninogenica ( P < 0.0001), and Candida dubliniensis ( P < 0.0001) presented significant differences between different groups. Means of total microbial count (×10 4 , ± SD ) for each group were recorded as follows: G1 (0.27 ± 2.04), G2 (0 ± 0), G3 (1.81 ± 7.50), and G4 (0.35 ± 1.81). Conclusions Differences in the geometry of implant connections and abutment screws have impacted the microbial leakage through the implant‐abutment interface. Implants attached with experimental conical‐head abutment screws showed lower counts of microorganisms when compared with conventional flat‐head screws.