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Identification of oral bacteria on titanium implant surfaces by 16S rDNA sequencing
Author(s) -
Melo Fabiana,
Nascimento Cássio,
Souza Diogo Onofre,
Albuquerque Rubens F.
Publication year - 2017
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12865
Subject(s) - firmicutes , bacteroidetes , actinobacteria , proteobacteria , fusobacteria , biology , biofilm , 16s ribosomal rna , dentistry , microbiology and biotechnology , bacteria , genetics , medicine
Objective To characterize the profile of microbial communities colonizing titanium implants with different surface treatments after exposure to the oral environment at the genus or higher taxonomic level. Material and methods Sixteen titanium disks, machined or sandblasted large‐grit and acid‐etched ( SLA ), were mounted on removable intraoral splints worn by four patients. After 24 h of intraoral exposure, biofilm samples were collected from disks and supra/subgingival teeth areas. The 16S rDNA genes from each sample were amplified, sequenced with the Miseq Illumina instrument and analyzed. Results A total of 29 genera and seven more inclusive taxa, representing the phyla Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria and candidate division TM 7 were identified in both titanium surfaces and teeth. No differences were found in relation to the operational taxonomic units ( OTU s) and microbial diversity, assessed by Chao 1 and Shannon indices, when comparing SLA and machined titanium surfaces. Conclusions Machined and SLA surfaces are colonized by similar numbers of prokaryotic OTUs after 24 h of exposure to the oral environment. Higher complexity of the titanium surface topography in the initial phase of biofilm maturation does not seem to significantly influence the colonizing microbiota.