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Comparison of bone morphogenetic protein‐2 delivery systems to induce supracrestal bone guided by titanium implants in the rabbit mandible
Author(s) -
Wen Bo,
Kuhn Liisa,
Charles Lyndon,
Pendrys David,
Shafer David,
Freilich Martin
Publication year - 2016
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12645
Subject(s) - implant , bone morphogenetic protein 2 , scaffold , dentistry , bone morphogenetic protein , chemistry , biomedical engineering , medicine , surgery , in vitro , biochemistry , gene
Objective To compare the ability of three non‐glycosylated/rh BMP ‐2 ( BMP ‐2) delivery systems to induce supracrestal bone. Material and methods Thirty‐six custom SLA ctive dental implants were partially embedded in transverse orientation into the posterior mandibles of 18 adult rabbits with the head of the implant located 3 mm supracrestal. Delivery of BMP ‐2 (30 μg) from the following materials was studied: (1) Ti implant + BMP ‐2 with hydroxyapatite ( HA )‐coated collagen (Col/ HA ) scaffold, (2) Ti implant with Col/ HA infused with PEG hydrogel + BMP ‐2, or (3) Ti implant with HA /β‐ TCP / PEG hydrogel scaffold + BMP ‐2. Scaffolds were secured with a metal “umbrella.” Non‐ BMP ‐2 contralateral controls were included. Micro CT imaging and histological analysis was performed after 10 weeks to assess new supracrestal bone formation. In vitro BMP ‐2 release studies were conducted. Results All treatment groups displayed new supracrestal bone formation. Ti + BMP ‐2 with Col/ HA (3.0 ± 0.2 mm) and Ti with Col/ HA / PEG hydrogel + BMP ‐2 (2.7 ± 0.4 mm) had significantly greater ( P < 0.05) outcomes than without BMP ‐2. Maximum bone volume occurred in the Ti implant with HA /β‐ TCP / PEG hydrogel scaffold + BMP ‐2 group. Conclusions The use of an implant system composed of a partially inserted Ti implant, adjacent scaffold and scaffold stabilizer resulted in the formation of new supracrestal bone across all test groups with and without BMP ‐2. Delivery of BMP ‐2 directly from the Ti implant increased bone height, BIC and bone volume as compared to no BMP ‐2 when a Col/ HA was used, but did not improve performance of the HA /β‐ TCP / PEG scaffold.