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Mechanical and hydrodynamic homecare devices to clean rough implant surfaces – an in vitro polyspecies biofilm study
Author(s) -
Ioannidis Alexis,
Thurnheer Thomas,
Hofer Deborah,
Sahrmann Philipp,
Guggenheim Bernhard,
Schmidlin Patrick R.
Publication year - 2015
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12436
Subject(s) - biofilm , chlorhexidine , anaerobic exercise , dentistry , implant , titanium , colony forming unit , chemistry , placebo , zoology , microbiology and biotechnology , medicine , surgery , biology , bacteria , physiology , pathology , genetics , alternative medicine , organic chemistry
Objectives To investigate the cleaning efficacy of a mechanical and a hydrodynamic homecare device on biofilm‐coated titanium surfaces with and without chlorhexidine. Material and methods Six‐species biofilms were grown on 108 SLA ‐titanium discs, which were cleaned as follows: sonic toothbrush alone (i) or in combination with either a 0.2% chlorhexidine (ii) or a placebo gel (iii) and oral irrigator (hydrodynamic action) with water (iv) or combined with 0.2% chlorhexidine solution (v). Untreated samples served as control (vi). Biofilms were then harvested either immediately after treatment (study part A) or after a regrowth phase of 24 h (study part B) and colony‐forming units ( CFU ) were assessed. Results were analysed using Whitney U ‐tests between the treatment groups. After the Bonferroni correction, the significance level was set at α = 0.0033. Results The median CFU counts directly after instrumentation accounted – in ascending order ( P ‐values in comparison with the control group A6 were <0.001 for all groups except for A3: P = 0.014) – 2.0E1 (A5), 1.1E5 (A4), 3.6E5 (A2), 3.3E5 (A1) and 6.8E6 (A3), respectively. The untreated control group showed the highest CFU counts: 1.8E7 (A6). After regrowth, the following CFU counts were measured in ascending order (all P ‐values <0.001 when compared to the control group B6 = 2.0E8): 1.6E2 (B5), 1.9E5 (B2), 1.4E7 (B4), 3.1E7 (B1) and 3.9E7 (B3). Conclusions An oral irrigator combined with 0.2% chlorhexidine is effective in reducing biofilms attached to rough titanium surfaces immediately after cleaning. Following a regrowth phase of 24 h, micro‐organisms could be equally effective removed with a sonic toothbrush combined with 0.2% chlorhexidine and an oral irrigator with 0.2% chlorhexidine.