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Oxygen plasma immersion ion implantation treatment enhances the human bone marrow mesenchymal stem cells responses to titanium surface for dental implant application
Author(s) -
Yang ChihHsiung,
Li YuChen,
Tsai WenFa,
Ai ChiFong,
Huang HerHsiung
Publication year - 2015
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12293
Subject(s) - biocompatibility , materials science , plasma immersion ion implantation , x ray photoelectron spectroscopy , protein adsorption , contact angle , mesenchymal stem cell , nuclear chemistry , titanium , chemistry , medicine , ion implantation , chemical engineering , pathology , ion , metallurgy , composite material , organic chemistry , engineering , polymer
Objectives The present investigation utilized a novel oxygen plasma immersion ion implantation (O‐ PIII ) treatment to create a dense and thin oxide layer on a titanium (Ti) surface for dental implant application. Materials and methods This study evaluated the behavior of human bone marrow mesenchymal stem cells ( hMSC s) on O‐PIII‐treated Ti. The O‐PIII treatments were performed using different oxygen ion doses (T L : 1 × 10 16 ; T M : 4 × 10 16 ; T H : 1 × 10 17 ions/cm 2 ). Results Analysis using an X‐ray photoelectron spectrometer ( XPS ) and high resolution X‐ray diffractometer ( HR‐XRD ) indicated that the O‐PIII‐treated specimen T M had the highest proportion of rutile phase TiO 2 component. The O‐PIII‐treated specimen T M had the greatest protein adsorption capability of the test Ti surfaces using XPS analysis and bicinchoninic acid ( BCA ) protein assay. Immunofluorescent staining revealed that h MSC s had the best cell adhesion on the O‐PIII‐treated specimen T M , whereas green fluorescent protein ( GFP )‐labeled h MSC s experienced the fastest cell migration based on a wound healing assay. Other assays, including MTT assay, Alizarin red S staining and Western blot analysis, demonstrated that the adhered h MSC s exhibited the greatest cell proliferation, mineralization, and differentiation capabilities on the T M specimen. Conclusions Oxidated Ti (primarily rutile TiO 2 ) was produced using a facile and rapid O‐ PIII treatment procedure, which enhances the biocompatibility of the Ti surface with potential implications for further dental implant application.