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Microbiological diversity of peri‐implantitis biofilm by S anger sequencing
Author(s) -
Silva Ennyo S. C.,
Feres Magda,
Figueiredo Luciene C.,
Shibli Jamil A.,
Ramiro Fernanda S.,
Faveri Marcelo
Publication year - 2014
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12231
Subject(s) - peri implantitis , biology , implant , biofilm , microbiology and biotechnology , medicine , gastroenterology , dentistry , zoology , genetics , surgery , bacteria
Background and objective To examine the microbial diversity associated with implants with or without peri‐implantitis and to identify differences between the bacterial communities in these clinical conditions. Material and methods Twenty subjects were assigned to a C ontrol group consisting of subjects with healthy implants and a Test group consisting of subjects with peri‐implantitis sites, as well as a healthy implant site ( n = 10/group). In the Test group, subgingival biofilm samples were taken from the deepest sites of the diseased implant. In both groups, samples were collected from one site around a healthy implant. DNA was extracted and the 16 S r RNA gene was amplified and sequenced, and the phylotypes were identified by comparison with known 16 S r RNA sequences. Results The phylogenetic identity of 1387 16 S r RNA gene clones was determined. Healthy implants demonstrated higher proportions of A ctinomyces, A topobium, G emella, K ingella and R othia and lower levels of C ampylobacter , D esulfobulbus, D ialister, E ubacterium, F ilifactor, M itsukella, P orphyromonas and P seudoramibacter ( M ann– W hitney U ‐test; P < 0.05). F usobacterium nucleatum , D ialister invisus , S treptococcus sp. human oral taxon (HOT) 064, F ilifactor alocis and M itsuokella sp. HOT 131 presented a higher mean proportion, while V eillonella dispar, A ctinomyces meyeri, G ranulicatella adiacens showed lower mean proportions in the peri‐implantitis sites when compared with healthy implants in both the C ontrol and T est groups ( M ann– W hitney U ‐test; P < 0.05). Conclusion Marked differences were observed in the composition of the subgingival biofilm between healthy and diseased implants. The biofilm associated with peri‐implantitis harbored more pathogenic bacterial species from the orange complex and other “unusual” putative pathogens, such as F . alocis , D . invisus and Mitsuokella sp. HOT 131.