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Photocatalytically induced hydrophilicity influences bone remodelling at longer healing periods: a rabbit study
Author(s) -
Hayashi M.,
Jimbo R.,
Xue Y.,
Mustafa K.,
Andersson M.,
Wennerberg A.
Publication year - 2014
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12138
Subject(s) - osseointegration , anatase , irradiation , titanium , in vivo , titanium dioxide , ultraviolet , photocatalysis , ultraviolet irradiation , rutile , nuclear chemistry , chemistry , implant , wound healing , biomedical engineering , materials science , biochemistry , surgery , organic chemistry , medicine , composite material , biology , catalysis , optoelectronics , nuclear physics , physics , microbiology and biotechnology
Objectives Previously, we have reported that photocatalytically active hydrophilicity of the anatase titanium dioxide (TiO 2 ) nanoparticles coated onto commercially pure titanium discs presented significantly improved hydrophilicity after ultraviolet irradiation. As hydrophilicity has shown enhancement of osseointegration, the in vivo responses were of great interest. The aim of this study was to evaluate whether or not the photo‐activated hydrophilicity generated at the time of implant placement has an effect on the longer healing periods for osseointegration. Materials and methods Photocatatytically active nanostructured TiO 2 powder (Degussa P‐25), which consists of approximately 80% anatase and 20% rutile, was spin‐coated onto commercially pure titanium discs and was heat‐treated thereafter. These P25‐coated discs were irradiated with ultraviolet ( UV ) light for the test (+ UV ) group, and non‐irradiated discs were prepared for the control (− UV ) group. Both groups of discs were placed in the rabbits' tibiae. After 12 weeks of healing period, histological analysis and gene expression analysis using real‐time RT ‐ PCR were performed. Results From the histological analyses, there were no specific differences between − UV and + UV groups. However, from the gene expression analysis, ALP , RUNX ‐2 and IL ‐10 were significantly upregulated for the + UV group compared with the − UV group. Conclusions The biologically enhancing effect to photocatalytically activated surfaces remained even after 12 weeks of healing time in terms of genetic responses.