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Morphological evidences of B io‐ O ss ® colonization by CD 44‐positive cells
Author(s) -
GalindoMoreno Pablo,
HernándezCortés Pedro,
AneirosFernández José,
Camara Miguel,
Mesa Francisco,
Wallace Stephen,
O'Valle Francisco
Publication year - 2014
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12112
Subject(s) - immunohistochemistry , osteocyte , pathology , sinus (botany) , neovascularization , bone canaliculus , maxillary sinus , osteolysis , chemistry , medicine , osteoblast , anatomy , biology , dentistry , angiogenesis , biochemistry , botany , in vitro , genus
Objectives Our objectives were to examine vascular and cellular colonization in anorganic bovine bone ( ABB ) after 6 months of healing in human maxillary sinus augmentation grafts. Material and methods Fifty unilateral maxillary sinus augmentation procedures were performed on 50 consecutive patients. Bone cores were obtained through the implant receptor sites 6 months later and evaluated with morphological image analysis and immunohistochemical techniques. Results Image analysis revealed a mean of 35.44 ± 16% vital bone, 31.66 ± 15% non‐mineralized tissue, and 32.72 ± 25% remnant ABB particles. In our patients, neovascularization in 46.3% of cases was demonstrated within ABB particles 6 months after sinus floor augmentation surgery. Neovascularization of ABB particles was inversely related to age, directly to osteoclast number per mm², and not influenced by habits or disease. CD 44‐positive cells colonization was found in 74% of cases. An important correlation was found regarding CD 44 expression and number of vessel in ABB particles ( r = 0.624 P < 0.001, Pearson). Osteopontin expression was detected on the interstitial boundary of bone with ABB particles and within the osteocyte lacunae and bone canaliculi and was relationship with presence of CD 44‐positive cells inside ABB particles ( r = 0.388, P = 0.046, Pearson). Conclusion In conclusion, images compatible with osteone colonized by osteocytes CD 44 positive and neovascularization in ABB particles were observed after 6 months of graft maturation. These biologic events have not previously been morphologically and immunohistochemically documented.