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Early biocompatibility of poly (ethylene glycol) hydrogel barrier materials for guided bone regeneration. An in vitro study using human gingival fibroblasts ( HGF ‐1)
Author(s) -
Dahlin Christer,
Johansson Anna,
Hoffman Maria,
Molenberg Aart
Publication year - 2014
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12076
Subject(s) - self healing hydrogels , biocompatibility , peg ratio , lactate dehydrogenase , viability assay , ethylene glycol , chemistry , polyethylene glycol , in vitro , tissue engineering , biophysics , biomedical engineering , biochemistry , polymer chemistry , enzyme , medicine , biology , organic chemistry , finance , economics
Objectives To evaluate the early cellular attachment and viability to modified polyethylene glycol ( PEG ) hydrogels with the influence of arginine‐glycine‐aspartic acid ( RGD ) in an in vitro model system. Material and methods Human gingival fibroblasts ( HGF ‐1) were cultured on 6 different modalities of PEG hydrogel in hydrophobic polystyrene wells. A total of 7500 cells/well (10000 cells/cm 2 ) were dispersed over the PEG filled wells and incubated in triplicates for 24 h, 7 and 13 days. Cell numbers were calculated by means of a N ucleo C ounter. Cell viability was determined by measuring lactate dehydrogenase ( LDH ). For statistical analysis, nonparametric K ruska– W allis test followed by D unetts T3 test were used. Results All PEG modifications showed good biocompatibility, as demonstrated by low LDH values per cell at the earlier two time points. After 13 days, all PEG modifications showed significantly lower number of cells compared with the controls, and the MX 60 configurations demonstrated significantly higher LDH /cell values compared with the other hydrogels. Conclusions Modifications of the physio‐chemical properties of PEG hydrogels and the addition of RGD and spacers influenced the initial cellular response of cultured HGF ‐1 cells. With the exception of MX 60 after 13 days, all PEG formulations performed similarly well. Early cellular response should be considered when developing PEG ‐based material for clinical purposes.